Prof. Dr. Dominik Paquet

Research focus: We are interested in the molecular and cellular mechanisms leading to neuronal death and cognitive decline in patients with neuropsychiatric disorders (e.g. Alzheimer's disease and Frontotemporal dementia) and neurovascular impairments (stroke and vascular cognitive impairment). Our main focus is on building advanced human in vitro model systems recapitulating these diseases using induced pluripotent stem cells and genome editing with CRISPR/Cas9.

Current GSN Students: Julien Klimmt, Angelika Dannert

Graduated GSN Students: Dr. Isabel Weisheit

Selected publications:

Kwart D*, Paquet D*, Teo S, Tessier-Lavigne M. Precise and efficient scarless genome editing in stem cells using CORRECT. Nature Protocols Feb;12(2):329-354 (2017). *equal first authors

Paquet D*, Kwart D*, Chen A, Sproul AA, Jacob S, Teo S, Olsen KM, Gregg A, Noggle S & Tessier-Lavigne M. Efficient introduction of specific homo- and heterozygous mutations with CRISPR/Cas9. Nature 533, 125–129 (2016). *equal first authors

Plucińska G*, Paquet D*, Hruscha A, Godinho L, Haass C, Schmid B, Misgeld T. In vivo imaging of disease-related mitochondrial dynamics in a vertebrate model system. Journal of Neuroscience 32, 16203–16212 (2012). *equal first authors

Paquet D, Bhat R, Sydow A, Mandelkow EM, Berg S, Hellberg S, Fälting J, Distel M, Köster RW, Schmid B, Haass C. A zebrafish model of tauopathy allows in vivo imaging of neuronal cell death and drug evaluation. Journal of Clinical Investigation 119, 1382–1395 (2009)

Exner N, Treske B, Paquet D, Holmström K, Schiesling C, Gispert S, Carballo-Carbajal I, Berg D, Hoepken HH, Gasser T, Krüger R, Winklhofer KF, Vogel F, Reichert AS, Auburger G, Kahle PJ, Schmid B, Haass C. Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin. Journal of Neuroscience 27, 12413–12418 (2007).